Product Information
| Description |
GAPDH mouse monoclonal |
| Protein full name |
Glyceraldehyde-3-phosphate dehydrogenase |
|
Synonyms |
GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase |
| Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse GAPDH |
| Isotype | IgG |
| Purity | Affinity purification |
| Predicted MW. | 37 kDa |
| Observed MW. | 37 kDa |
| Subcellular location |
Cytoplasm,Nucleus,Membrane |
| Uniprot ID |
Applications
| Applications |
WB |
IHC/IF | |
| Species |
Human,Rat,Mouse |
Human,Rat,Mouse |
|
| Dilution | 1:1000 |
1:200 |
|
| Positive tissue | Hela,HepG2,jurkat,lung,kidney,brain | Liver cancer,brain |
Background
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.In sperm, a testis-specific isoenzyme GAPDHS is expressed.
Images
 |
Western blot analysis of GAPDH (GB12002) at dilution of 1:1000. Lane 1:Hela cell lysate Lane 2:HepG2 cell lysate Lane 3:Jurkat cell lysate Lane 4:Mouse lung tissue lysate Lane 5:Mouse kidney tissue lysate Lane 6:Mouse brain tissue lysate Lane7: Rat lung tissue lysate Lane 8:Rat kidney tissue lysate Lane 9:Rat brain tissue lysate |
 |
Immunohistochemistry analysis of paraffin-embedded human liver cancer using GAPDH (GB120002) at dilution of 1:200 |
 |
Immunohistochemistry analysis of paraffin-embedded mouse brain using GAPDH (GB120002) at dilution of 1:200 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
