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Anti - ZO1 tight junction protein Rabbit pAb

货号:GB111402

品牌:Servicebio

价格:¥650

规格: 100 μl

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Product Information

 

 

Description

ZO1 tight junction protein rabbit polyclonal

Protein full name Tight junction protein ZO-1

Synonyms

TJP1, ZO-1, Tight junction protein 1, TJP1,
Zonula occludens protein 1

Immunogen KLH conjugated Synthetic peptide
corresponding to Human ZO-1
Isotype IgG
Purity Affinity purification
Subcellular location Cell membrane
Predicted MW. 195 kDa
Observed MW. 220 kDa
Uniprot ID
Q07157P39447

 


 

Applications

 

 

Applications WB,IF
Species Human, Mouse,Human, Mouse, Ra
Dilution 1: 500-1: 1000,1: 500-1: 1000
Positive Tissue kidney, spleen,kidney cancer, jejunum, small intestine

 


 

Background

Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium. ZO-1 is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells . It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin . ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation.
 


 

Images

 

Western blot analysis of ZO-1 (GB111402)
at dilution of 1: 1000
Lane 1: HEK293 cell lysate
Lane 2: HT29 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: A431 cell lysate
Lane 6: Mouse kidney tissue lysate
Lane 7: Mouse spleen tissue lysate


 

Immunofluorescent analysis of paraformaldehyde-fixed human kidney cancer using ZO-1 (GB111402) at dilution of 1: 800
 

Immunofluorescent analysis of paraformaldehyde-fixed mouse jejunum using
ZO-1 (GB111402) at dilution of 1: 800
 

Immunofluorescent analysis of paraformaldehyde-fixed mouse small intestine using ZO-1 (GB111402) at dilution of 1: 800
 

Immunofluorescent analysis of paraformaldehyde-fixed rat small intestine using ZO-1 (GB111402) at dilution of 1: 800
 


 

Storage

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. 

 


 

NOTE: This product is intended for research only.